New Qormi striker Terence Vella joined the squad for the trip to Italy as he replaced the injured Dylan Grima. The Balzan captain pulled out of the squad after sustaining a knee injury in training.
National coach Pietro Ghedin had initially dropped Vella from the provisional squad along with Sliema goalkeeper Glenn Zammit, Pembroke defender Alex Cini and Tarxien midfielder Ayrton Azzopardi.
Clifford Gatt Baldacchino of Sliema had earlier replaced Jonathan Caruana who sustained an injury in the clash between Valletta and Sliema.
The players who travel to Italy on Wednesday are:
goalkeepers Henry Bonello (Valletta), Justin Haber (Birkirkara), Andrew Hogg (Kalloni);
defenders Andrei Agius (Hibs), Steve Borg (Aris Limassol), Ryan Camilleri (Valletta), Clifford Gatt Baldacchino (Sliema), Alex Muscat (Sliema), Zach Muscat (Birkirkara), Joseph Zerafa (Birkirkara), Clayton Failla (Hibs);
midfielders Roderick Briffa (Valletta), Andrew Cohen (Hibs), Paul Fenech (Birkirkara), Bjorn Kristensen (Hibs), Rowen Muscat (Birkirkara), Steve Pisani (Floriana), Gareth Sciberras (Birkirkara);
forwards Alfred Effiong (Balzan), Michael Mifsud (Sliema), Jean Pierre Mifsud Triganza (Valletta), Andre Schembri (Omonia Nicosia), Terence Vella (Qormi).