The draws for the Round of 16 of the BOV Challenge Cup were held at the Centenary Hall on Wednesday.
This new competition is open for clubs from the BOV Challenge League.
Matches will be played between February 25 and 27 apart from the clash between Valletta and Senglea Athletic which will be played on March 12.
| Tue 25-Feb-2025 | Pieta’ Hotspurs FC vs Fgura United FC | 20:30 | Victor Tedesco Stadium |
| Wed 26-Feb-2025 | St. Andrews FC vs Marsa FC | 18:15 | Centenary Stadium |
| Wed 26-Feb-2025 | Sirens FC vs Tarxien Rainbows FC | 20:30 | Victor Tedesco Stadium |
| Wed 26-Feb-2025 | Lija Athletic FC vs Gudja United FC | 20:45 | Centenary Stadium |
| Thu 27-Feb-2025 | Zurrieq FC vs Swieqi United FC | 18:00 | Centenary Stadium |
| Thu 27-Feb-2025 | Mgarr United FC vs Mtarfa FC | 20:30 | Centenary Stadium |
| Thu 27-Feb-2025 | Zebbug Rangers FC vs Santa Lucia FC | 20:30 | Victor Tedesco Stadium |
| Wed 12-Mar-2025 | Valletta FC vs Senglea Athletic FC | 20:15 | Centenary Stadium |